Hyperspectral in-vivo two-photon microscopy of intrinsic fluorophores
نویسندگان
چکیده
In-vivo two-photon imaging of intrinsic fluorescence allows metabolic function to be evaluated on a cellular level. A method of validating, identifying and separating the fluorophores present in an in-vivo two-photon image is described. ©2008 Optical Society of America OCIS codes: (170.0170) Medical optics and biotechnology; (110.4234) Multispectral and hyperspectral imaging; (170.2655) Functional monitoring and imaging; (170.6280) Spectroscopy, fluorescence, and luminescence;
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